Endogenous SLC39A1 protein expression in K562 erythroleukemia cells and the effects of Zn2+ depletion. K562 erytholeukemia cells without (A) or with (B) a 1 hour preincubation in 10 uM TPEN prior to being labeled with SLC39A1 (hZIP1)antibodies and visualization with epifluorescence. (C) - 65Zn2+ uptake measured in K562 cells with (circle) or without (square) preincubation with 10 uM TPEN. Each point represents the mean +/- SEM of triplicate determinations.
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